Anand Lab

2023

Belan O, Greenhough L, Kuhlen L, Anand R, Kaczmarczyk A, Gruszka DT, Yardimci H, Zhang X, Rueda DS, West SC and Boulton S J (2023) Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein. Mol Cell, 83:2925–2940.e8 Link

Greenhough LA, Liang CC, Belan O, Kunzelmann S, Maslen S, Rodrigo-Brenni MC, Anand  R, Skehel M, Boulton SJ and West SC (2023) Structure and function of the RAD51B–RAD51C–RAD51D–XRCC2 tumour suppressor. Nature, 619:650–657 Link

Fleury H, MacEachern MK, Stiefel CM, Anand R, Sempeck C, Nebenfuehr B, Maurer-Alcalá K, Ball K, Proctor B, Belan O, Taylor E, Ortega R, Dodd B, Weatherly L, Dansoko D, Leung JW, Boulton SJ and Arnoult N (2023) The APE2 nuclease is essential for DNA double-strand break repair by microhomology-mediated end joining. Mol Cell, 83:1429–1445.e8. Link

2022

Belan O, Sebald M, Adamowicz M, Anand R, Vancevska A, Neves J, Grinkevich V, Hewitt G, Segura-Bayona S, Bellelli R, Robinson HMR, Higgins GS, Smith GCM, West SC, Rueda DS and Boulton SJ (2022) POLQ seals post-replicative ssDNA gaps to maintain genome stability in BRCA-deficient cancer cells. Mol Cell, 82:4664–4680.e9 Link

Anand R, Buechelmaier E, Belan O, Newton M, Vancevska A, Kaczmarczyk A, Takaki T, Rueda DS, Powell SN and Boulton SJ (2022) HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51. Nature, 601:268–273 Link

Halder S, Sanchez A, Ranjha L, Reginato G, Ceppi I, Acharya A, Anand R and Cejka P (2022) Double-stranded DNA binding function of RAD51 in DNA protection and its regulation by BRCA2. Mol Cell, 82:3553–3565.e5 Link

2021

Belan O, Anand R and Boulton SJ (2021) Mechanism of mitotic recombination: insights from C. elegans. Curr Opin Genet Dev, 71:10–18 Link

Belan O, Moore G, Kaczmarczyk A, Newton MD, Anand R, Boulton SJ and Rueda DS (2021) Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis. STAR Protoc, 2:100588 Link

Belan O, Barroso C, Kaczmarczyk A, Anand R, Federico S, O’Reilly N, Newton MD, Maeots E, Enchev RI, Martinez-Perez E, Rueda DS and Boulton SJ (2021) Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins. Mol Cell, 81:1058–1073.e7 Link

Sharma S, Anand R, Zhang X, Francia S, Michelini F, Galbiati A, Williams H, Ronato DA, Masson JY, Rothenberg E, Cejka P and d’Adda di Fagagna F (2021) MRE11-RAD50-NBS1 complex is sufficient to promote transcription by RNA polymerase II at double-strand breaks by melting DNA ends. Cell Rep, 34:108565 Link 

2020

Cannavo E, Sanchez A, Anand R, Ranjha L, Hugener J, Adam C, Acharya A, Weyland N, Aran-Guiu X, Charbonnier JB, Hoffmann ER, Borde V, Matos J and Cejka P (2020) Regulation of the MLH1–MLH3 endonuclease in meiosis. Nature, 586:618–622 Link

Huang JW, Acharya A, Taglialatela A, Nambiar TS, Cuella-Martin R, Leuzzi G, Hayward SB, Joseph SA, Brunette GJ, Anand R, Soni RK, Clark NL, Bernstein KA, Cejka P and Ciccia A (2020) MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage. Nat Commun, 11:2948 Link

Howard SM, Ceppi I, Anand R, Geiger R and Cejka P (2020) The internal region of CtIP negatively regulates DNA end resection. Nucleic Acids Res, 48:5485–5498 Link

Ceppi I, Howard SM, Kasaciunaite K, Pinto C, Anand R, Seidel R and Cejka P (2020) CtIP promotes the motor activity of DNA2 to accelerate long-range DNA end resection. Proc Natl Acad Sci USA, 117:8859–8869 Link

2019

Kasaciunaite K, Fettes F, Levikova M, Daldrop P, Anand R, Cejka P and Seidel R (2019) Competing interaction partners modulate the activity of Sgs1 helicase during DNA end resection. EMBO J, 38:e101516 Link

Anand R, Jasrotia A, Bundschuh D, Howard SM, Ranjha L, Stucki M and Cejka P (2019) NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation. EMBO J, 38:e101005 Link

2018

Anand R, Pinto C and Cejka P (2018) Methods to study DNA end resection I: recombinant protein purification. Methods Enzymol, 600:25–66 Link

Pinto C, Anand R and Cejka P (2018) Methods to study DNA end resection II: biochemical reconstitution assays. Methods Enzymol, 600:67–106 Link

2017

Taglialatela A, Alvarez S, Leuzzi G, Sannino V, Ranjha L, Huang JW, Madubata C, Anand R, Levy B, Rabadan R, Cejka P, Costanzo V and Ciccia A (2017) Restoration of replication fork stability in BRCA1- and BRCA2-deficient cells by inactivation of SNF2-family fork remodelers. Mol Cell, 68:414–430.e8 Link

Daddacha W, Koyen AE, Bastien AJ, Head PE, Dhere VR, Nabeta GN, Connolly EC, Werner E, Madden MZ, Daly MB, Minten EV, Whelan DR, Schlafstein AJ, Zhang H, Anand R, Doronio C, Withers AE, Shepard C, Sundaram RK, Deng X, Dynan WS, Wang Y, Bindra RS, Cejka P, Rothenberg E, Doetsch PW, Kim B and Yu DS (2017) SAMHD1 promotes DNA end resection to facilitate DNA repair by homologous recombination. Cell Rep, 20:1921–1935 Link

 Kim JH, Grosbart M, Anand R, Wyman C, Cejka P and Petrini JHJ (2017) The Mre11-Nbs1 interface is essential for viability and tumor suppression. Cell Rep, 18:496–507 Link

2016

Anand R, Ranjha L, Cannavo E and Cejka P (2016) Phosphorylated CtIP functions as a co-factor of the MRE11-RAD50-NBS1 endonuclease in DNA end resection. Mol Cell, 64:940–950 Link

Lu H, Shamanna RA, Keijzers G, Anand R, Rasmussen LJ, Cejka P, Croteau DL and Bohr VA (2016) RECQL4 promotes DNA end resection in repair of DNA double-strand breaks. Cell Rep, 16:161–173 Link

2014

Ranjha L, Anand R and Cejka P (2014) The Saccharomyces cerevisiae Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to holliday junctions. J Biol Chem, 289:5674–86 Link